Explore Cloning Services

In the ever-evolving landscape of molecular biology, the need for precise, efficient, and reliable genetic tools has never been greater. Whether you're exploring the intricacies of gene expression, modifying genetic sequences, or aiming to silence specific genes, our suite of services is designed to empower your research. Our specialized offerings include:

  • Molecular Cloning Services

    Maximize your lab efficiency by tapping into our deep expertise in AAV, adenovirus, and lentivirus molecular cloning services. Our comprehensive approach covers everything from the design and construction of custom viral vectors to their packaging and purification. With our expertise in gene synthesis and mutagenesis and extensive collection of over 17,000 human ORFs, you can utilize a diverse range of tags, reporters, and inducible systems to fine-tune your viral vectors for your specific research endeavors.

  • Gene Synthesis and Mutagenesis

    Save time and achieve precise genetic modifications tailored to your exact specifications with our gene synthesis and site-directed mutagenesis services. Our dedicated team manages the entire process, from gene synthesis or mutation to cloning into diverse vector backbones, culminating in the packaging and purification of viral particles for both in vitro and in vivo applications. Whether you're supplying a specific sequence or choosing from our vast collection of human ORFs, we're here to ensure your genetic modifications are executed flawlessly.

  • shRNA Cloning

    Unlock streamlined, time-saving, and cost-effective solutions for your gene silencing experiments with our cutting-edge shRNA Cloning Services. Our diverse shRNA cloning options are tailored to your needs, with a typical recommendation to initiate testing using shRNAs in a viral vector backbone. Leveraging our deep expertise, we confidently promise a minimum of 70% knockdown for your targeted gene through our precision-designed shRNA constructs.

    After pinpointing the optimal shRNA, we can adeptly package it into AAV shRNA, adenovirus shRNA, or lentivirus shRNA viral particles for maximum gene knockdown efficiency. Additionally, our innovative technology can express up to four shRNA sequences in a single vector, and we proudly offer specialized miR-based and Cre-ON shRNA constructs to further enhance your research capabilities

Elevate Your Research with Global Cloning and CGT Expertise

Harness our worldwide reach and proficiency. Boasting over 110 facilities across 20+ countries, we're committed to propelling your custom cloning objectives forward with our cell and gene therapy (CGT) CDMO solutions. Alongside our viral vector packaging services, we provide a catalog of viral vector products, encompassing control viruses and AAV reference materials.

Our suite of services encompasses:

Our integrated CGT development platform expedites your market trajectory, adeptly navigating the complexities of viral packaging. With over 75 years of scientific expertise, we guide your projects from Phase I CGMP manufacturing directly to large-scale commercial output. Our approach prioritizes systematic commercial readiness, ensuring reproducibility and scalability. By partnering with us, you gain a strategic edge, navigating the nuanced challenges of late-phase Chemistry, Manufacturing, and Controls (CMC) in CGT manufacturing and optimizing the swift market introduction of your therapeutic modalities.

Simplified Custom Cloning Process

With three straightforward steps, you get a streamlined and efficient cloning experience, setting the foundation for your research success.

  1. Gene Sequence Submission: Start by providing us with your desired gene sequence. Our team is on standby to assist if you need guidance or modifications.
  2. Promoter Specification: Next, specify your preferred promoter to optimize your gene expression. We’re here to provide recommendations if you need them.
  3. Vector Backbone Selection: Finally, choose from our diverse range of vector backbones, including but not limited to AAV, lentiviral, and adenoviral vectors. If you’re unsure, our experts can recommend the best fit.

As we navigate the cloning process we will provide you with updates at every crucial juncture. After cloning, stringent quality assurance is performed to ensure your construct aligns perfectly with your specifications. When you receive your tailored plasmids or viral vectors, know that our support continues, assisting you in seamlessly integrating them into your research.  

Frequently Asked Questions (FAQs) About Cloning Services

  • Are there any limitations to which ORFs can be shuttled?

    If you want to generate viral particles, you must not exceed the packaging capacity of the virus. Please note that the packaging capacity refers to the total insert size between and including the ITRs or LTRs.

    • AAV cloning capacity: 4.7 kb
    • Adenovirus cloning capacity: 7.5 kb
    • Lentivirus cloning capacity: 6 kb
  • What information do I need to provide for gene synthesis services?

    For gene synthesis services, please provide the following:

    • Gene ID/Name (specify variant, if applicable) or file containing the desired sequence
    • Name of desired viral vector backbone or name of customer-provided vector
  • What information do I need to provide for mutagenesis services?

    For mutagenesis services, please provide the following:

    • Gene ID/Name (specify variant, if applicable) or file containing the desired sequence
    • Description of sites/base pairs to mutate
    • Name of desired viral vector backbone or name of customer-provided vector
    • If you are sending your own vector for mutating or cloning, please send us at least 5 ug and a sequence/plasmid map
  • Does Charles River validate shRNA sequences?

    We do not validate shRNA sequences designed as a part of the shRNA molecular cloning services. However, we will send you the shRNA plasmids to test. Once you determine which shRNA construct works best for your experiments, we can package your selected shRNA construct into virus.